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. 2019 Jan 30;9:958. doi: 10.1038/s41598-018-35757-x

Figure 5.

Figure 5

Analysis of miRs-34c-5p and 155 in Meth treated CD4+ T-cells: Unstimulated (A) and Stimulated (B) CD4+ T-cells were treated with Meth (100 µM) for 3 days, cells were harvested on days 0, 1 and 3 (D0, D1 and D3), RNA was isolated, and the expression of miRs-34c-5p and 155 was analyzed by qRT-PCR by normalizing all the samples to untreated samples (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001). (C) Expression analyses of miRs-34c-5p and 155 by qRT-PCR in untreated and Meth treated unstimulated CD4+ T-cells in the presence or absence of sigma-1 receptor inhibitor (σ1 R inh.) at day 3 (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001).