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. 2019 Jan 30;10:492. doi: 10.1038/s41467-018-08283-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Screening and validation of Sk-CRM4 fragments. a In vivo bioluminescence imaging at week 2 and week 4 post vector injection and quantification of luciferase expression from the different individual organs of adult CB17/IcrTac/Prkdc^scid mice injected intravenously with scAAV9-Des-Luc2 (control, no Sk-CRM), scAAV9-Sk-CRM4^a-Des-Luc, scAAV9-Sk-CRM4^b-Des-Luc, scAAV9-Sk-CRM^c-Des-Luc, scAAV9-Sk-CRM4^d-Des-Luc, and scAAV9-Sk-CRM4^e-Des-Luc vectors at a dose of 1 × 10¹⁰ vg per mouse. The corresponding color scale from 6.70e + 03 (blue) ph/s/cm²/sr to 2.70e + 05 (red) ph/s/cm²/sr was shown. Photon emission was measured dynamically during 7 min in a supine position. b Ex vivo bioluminescence imaging of individual organs such as the heart and the different muscle groups harvested at 6 weeks post vector injection was represented on a color scale with luciferase intensities ranging from 5.92e + 03 (blue) ph/s/cm²/sr to 5.08e + 04 (red) ph/s/cm²/sr. The bioluminescence signal was quantified for 5 min. c Luciferase expression from the individual tissues was measured as total flux, expressed in photons/sec/cm²/sr. The fold-difference of the total flux was indicated for each Sk-CRM4 fragments relative to that of the control group containing the Des promoter without any Sk-CRM. The total flux data were displayed as mean + s.e.m. (n = 3)