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. 2019 Jan 30;9:996. doi: 10.1038/s41598-018-37650-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overview of media used for DE differentiation of hPSCs. DE differentiation of hPSCs was performed according to the CA-A approach⁴, but with different basal media and supplements. Media formulations were changed step-wise. First, FCS was replaced by a modified B-27 (mB-27) to obtain chemically defined conditions. Second, advanced RPMI 1640 was replaced by MCDB131 or RPMI 1640 for BSA-free conditions. Third, mB-27 was replaced by mB-27XF to obtain a fully xenogeneic-free condition.