Table 1.
Composition of mB-27 and mB-27XF.
| component | concentration in mB-27(XF) [µg/mL] |
|---|---|
| Biotin | 5 |
| L-Carnitine | 100 |
| Ethanolamine | 50 |
| D-Galactose | 750 |
| Putrescine | 805 |
| Glutathion (red.) | 50 |
| Transferrin | 250 |
| Sodium selenite | 0,72 |
| Corticosterone | 1 |
| Linoleic Acid | 50 |
| Linolenic Acid | 50 |
| Progesterone | 0,32 |
| Retinylacetate | 5 |
| Tocopherol | 50 |
| Tocopherol acetate | 50 |
| Triiodothyronine | 0,1 |
| Catalase | 125 |
| Superoxide dismutase | 125 |
mB-27 was prepared as described previously28. Bovine catalase and bovine superoxide dismutase (written in italic) that are present in mB-27 were omitted from the formulation of mB-27XF to obtain a xenogeneic-free medium supplement. mB-27 and mB-27XF were applied at a dilution of 1:100 for supplementation of differentiation media.