Table 1.

Codes and details of larval culturing and comparisons performed in molecular analyses, plus the number of annotated transcripts up-regulated in the treated samples when searched against the (A) GenBank non-redundant and (B) Swissprot human databases.

Time	Treatment		A: GenBank non-redundant	B: Swissprot human
2 days	Ambient adults/low pH larvae	CE2	204	91 (81)
	Low pH for both adults and larvae	E2	274	117 (109)
3 days	Ambient adults/low pH larvae	CE3	92	49 (43)
	Low pH for both adults and larvae	E3	355	153 (138)
4 days	Ambient adults/low pH larvae	CE4	469	315 (309)
	Low pH for both adults and larvae	E4	1356	764 (659)

Unique matches against Swissprot are in brackets. Treatments: In the experimental manipulations the “C” signifies ambient or control conditions of ~400 µatm; the “E” signifies low pH (~1000 µatm, denoted “E”). CE: Adults cultured under ambient conditions with larvae spawned into low pH (samples CE2-CE4); E: larvae spawned into low pH produced from adults also cultured at low pH (samples E2-E4). For the molecular analyses, the transcriptional profiles of all treated larvae were compared against a set of age-matched larvae spawned into ambient conditions produced from ambient cultured adults.