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. 2019 Jan 30;9:977. doi: 10.1038/s41598-018-37403-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hepatic steatosis is ameliorated in HFD-fed BAFF^−/− mice. (A) Representative H&E staining of livers from mice after 24 weeks of ND or HFD consumption (scale bar, 500 μm). (B) Representative OsO₄ staining (left) and quantification of the OsO₄-positive areas (right) of livers (scale bar, 500 μm; images from eight different fields; n = 8/group). (C) Hepatic TG and (D) total cholesterol levels (n = 5/group). For all bar plots, data are expressed as the mean ± SEM, and significance was determined by the Mann–Whitney U test. *P < 0.05 and ***P < 0.001. BAFF, B cell-activating factor; WT, wild-type; ND, normal diet; HFD, high-fat diet; TG, triglyceride.