Fig. 1.

AZD9496 is comparable to fulvestrant in endocrine-sensitive ER +  cells. a Cell growth assay of T47D, MCF7, ZR75-1, 600MPE, and MDA-MB-415 parental cells treated for 6 days with different endocrine treatments. b Immunoblot for ER expression and signalling (PR) in MCF7 cells in the presence of 5% cs-FBS (ED-medium, ED), ED+E2, or 10% FBS and two different concentrations 10^–6 M (–6) and 10^–7 M (–7) of fulvestrant or AZD9496. c mRNA levels of progesterone receptor (PGR) in MCF7 cells were assessed using real-time quantitative PCR (RT qPCR). The mRNA expression was normalised to the actin housekeeping gene, and expression levels are presented as –ΔΔCT compared with E2 control. d ERE-luciferase activity assay in MCF7 cells treated by ED, ED+E2, or 10% FBS for 24 h. SEM are shown (n = 3); **p < 0.01; ***p < 0.001; ****p < 0.0001