Fig. 3. Downregulation of cyclinB1 triggered autophagy via AMPK-ULK1-dependent signal pathway.

a The Kyoto Encyclopedia of Genes and Genomes pathway analysis of differentially expressed genes (DEGs) in siNC- and siCB1-treated cells. b Gene Ontology analysis of the genes related to cyclinB1 knockdown in CNE-1 and CNE-2 cells. c Western blot analysis of proteins related to autophagy signal pathway. d Real-time PCR analysis of proteins related to autophagy signal pathway. e N-acetylcysteine 10 mM remarkably attenuated AMPK phosphorylation and LC3-II level in cyclinB1 knockdown cells. f Double knockdown of AMPK and cyclinB1 evidently abrogated cyclinB1 silencing-induced autophagy. All data represented mean ± s.d. from three independent experiments; P values were calculated in comparison with cells treated with siNC (control) unless indicated. NS: P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001