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. 2019 Jan 2;42(1):17–27. doi: 10.14348/molcells.2018.0329

Fig. 4. USP44 knockdown decreases the tumorigenic abilities of prostate cancer cell lines.

Fig. 4

(A) Expression of E-cadherin, β-catenin, and vimentin in USP44 knockdown stable cell lines was examined by immunoblotting. (B) Wound healing assays of USP44 knockdown stable cell lines. Monolayers of stable cell lines were scratched with a yellow micropipette tip. Values are expressed as the mean ± SD of three independent experiments. (C) Migration assays of USP44 knockdown stable cell lines. Equal amounts of cells from the stable cell lines were seeded into the transwell migration chamber. After 22 h, the number of cells that had migrated to the lower chamber was counted. Values are expressed as the mean ± SD of three independent experiments. (D) Matrigel invasion assays of USP44 knockdown stable cell lines. Equal numbers of cells from the stable cell lines were seeded into the matrigel-coated chamber. After 22 h, the invaded cells on the lower surface of the membrane were counted. Values are expressed as the mean ± SD of three independent experiments. The p value is shown from a Student’s t-test. *p < 0.05, **p < 0.01