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. 2018 Dec 5;42(1):67–78. doi: 10.14348/molcells.2018.0255

Fig. 1. HBx activates DNMTs during HBV infection.

Fig. 1

HepG2 cells were transiently transfected with either 1.2-mer WT HBV replicon or its HBx-null derivative for 48 h. For lane 4, HBx-expression plasmid was included. (A, C, F) Levels of the indicated proteins were determined by Western blotting. (B) Levels of HBV particles released from the cells were determined by IP-coupled conventional PCR and real-time PCR (n = 5). (D) DNMT activity was measured as described in Methods (n = 4). (E) MSP analysis was performed to determine whether CpG islands in the promoters of RAR-β and E-cadherin are methylated (M) or unmethylated (U) in the presence or absence of HBx.