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. 2019 Jan;60(1):71–78. doi: 10.2967/jnumed.118.212720

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© 2019 by the Society of Nuclear Medicine and Molecular Imaging.

Immediate Open Access: Creative Commons Attribution 4.0 International License (CC BY) allows users to share and adapt with attribution, excluding materials credited to previous publications. License: https://creativecommons.org/licenses/by/4.0/. Details: http://jnm.snmjournals.org/site/misc/permission.xhtml.

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FIGURE 5. — (Top 2 rows) PSMA immunohistochemistry of mouse tissue sections (paraffin embedded, 2 μm): first row, whole organ; second row, kidney and tumor 10× magnification and spleen 20× magnification. (Bottom 2 rows) Tissue distribution of PSMA-I&F (2 nmol, 1 h after injection) observed by Sulfo-Cy5 fluorescence microscopy of mouse tissue cryosections (10 μm): third row, 4× magnification; fourth row, 20× magnification. Please note that images have been scaled to comparable visual fluorescence intensity. Exposition times during fluorescence microscopy: 2 s for kidney, 12 s for spleen and tumor, respectively.