Figure 4.

SWEET11 and 12 Protein Colocalization in Response to P. Brassicae Infection.

The presence of SWEET11-GUS and SWEET12-GUS fusion proteins in hypocotyls was studied by β-Glucuronidase activity assay followed by embedding in Technovit and sectioning. Shown are 10-μm Technovit embedded and Safranin-O counterstained sections. A and B, SWEET11:SWEET11-GUS (A) and SWEET12:SWEET12-GUS (B), both 16 DAI and 26 DAI with (Inf) and without (Mock) P. brassicae infection. Insets show representative phloem bundles from regions marked with squares on larger images of radial hypocotyl sections. Scale bars = 200 μm for radial sections and 20 μm for insets.