Figure 5.

P. brassicae Disease Development Progression in the sweet11;12 Double Mutant and Single Gene Complementation Lines.

(A)Toluidine blue stained 10-μm hypocotyl sections of Col-0, sweet11;12 and sweet11;12 SWEET11, sweet11;12 SWEET12 complementation lines at 16 DAI and 26 DAI. Scale bar =200 μm. RS, resting spores; MP, multinuclear plasmodia.

(B) Gall development in sweet11;12 mutants. Mean hypocotyl widths of 14 or 28 replicates calculated using a mixed linear model are plotted along with the se. Different letters indicate significant differences between means (Benjamini-Hochburg adjusted P value < 0.05).

(C) Hypocotyl width increase induced by P. brassicae infectioncalculated from (B). Asterisks denote a significant difference between mutant and wild type (P < 0.05).