Table 1. Candidate Avenacin Glycosyltransferase Genes.
| A. strigosa UGT Genes | Group | Gene Expression Profilesa | Protein Level Ratio (RT/EZ)b | |||
|---|---|---|---|---|---|---|
| RT | EZ | WR | L | |||
| AsUGT98B4 | D | +++ | + | ++ | ++ | ND in EZ |
| AsUGT701A5 | D | +++ | + | ++ | − | ND |
| AsUGT99C4 | D | ++ | − | + | − | ND in EZ |
| AsUGT99D1 | D | +++ | − | ++ | − | 2.30 |
| AsUGT99B9 | D | +++ | ++ | ++ | ++ | 0.78 |
| AsUGT99A6 | D | ++ | − | ++ | − | ND in RT |
| AsUGT705A4 | — | +++ | − | ++ | − | 0.77 |
| AsUGT706F7 | E | +++ | + | ++ | + | 1.34 |
| AsUGT93B16 | O | +++ | + | +++ | +++ | 1.70 |
| AsUGT74H5 | L | +++ | − | ++ | − | 5.56 |
RT, root tip; EZ, elongation zone; WR, whole root; L, leaves; ND, not detected. “—” indicates an absence of amplification; “+,” “++,” and “+++” indicate the relative intensity of the amplified fragment on the gel based on the housekeeping gene GAPDH.
a
Gene expression profiles were obtained by RT-PCR (Figure 2B).
b
Based on proteomic analysis (Supplemental Figure 1) and displayed as a ratio of protein detected between RT and EZ.