Figure 10.

Effect of SlMYC1 Downregulation on JA-Induced Expression of Terpene Synthase Genes.

Relative transcript levels of Jasmonate-inducible protein-21 (JIP-21) and terpene synthase genes in stem trichomes and leaves of (A) wild-type (WT) Moneymaker and ir-SlMYC1 line 8, 18, and 25 and (B) wild-type RZ (WT-RZ-1), heterozygous MYC1/myc1 (Het), and homozygous myc1 (Hom) plants. The plants were sprayed with JA solution (1 mM JA and 0.05% SilwetL-77 in tap water) or control solution (0.05% SilwetL-77 in tap water) and samples were collected 24 h later. Transcript levels were determined by qRT-PCR. Bars represent mean values (±se) of three to four biological replicates normalized to Actin transcript levels. Bars annotated with different letters were significantly different according to Fisher’s LSD test (P ≤ 0.05) after ANOVA.