Analysis of SI Behavior of T0 Plants Carrying Either a 1-bp Deletion or a 1-bp Insertion Frame-Shift Allele of S2-SLF1.
(A) Aniline blue staining of pollen tubes in the bottom segment of the style after a wild-type S3S3 plant was separately pollinated by pollen from T0 plants, #2/S2*S3 and #35/S2*S3, and a wild-type S2S3 plant. White arrows indicate where growth of most pollen tubes stopped, in the case of incompatible pollinations. Scale bar = 1 mm.
(B) Progeny analysis of crosses using pollen from #2/S2*S3 or #35/S2*S3 to separately pollinate the wild-type S7S7 and S13S13 pistils. n indicates the number of plants in each progeny analyzed.