Figure 6.
Assessment of Interaction between S2-SLF1 and S12-RNase by in Vivo Gain-of-Function Assay.
(A) Analysis of 24 T2 plants resulting from the cross, S2S12 x LAT52P:S2-SLF1:GFP/S2S12. T1 indicates genomic DNA from the T1 plant LAT52P:S2-SLF1:GFP/S2S12; S2S3 indicates genomic DNA from a wild-type S2S3 plant (as negative control for the PCR amplification of the GFP transgene and S12-RNase gene); S3S12 indicates genomic DNA from a wild-type S3S12 plant (as negative control for the PCR amplification of the S2-RNase gene).
(B) Chi-square analysis of the S-haplotype inheritance in the 24 T2 plants analyzed in (A). Chi-square was used to test the null hypothesis of S-haplotype inheritance, 1:1 ratio of S2S12:S12S12 versus 1:2:1 ratio of S2S2:S2S12:S12S12.