Figure 7.
Analysis of SI Behavior of S2* Pollen Carrying a 3-bp or a 6-bp In-Frame Deletion Allele of S2-SLF1.
(A) Pollen tube growth in the style after a wild-type S3S3 plant was separately pollinated with pollen from T0 plants #13/S2*S3 (carrying a 3-bp deletion allele) and #27/S2*S3 (carrying a 6-bp deletion allele).
(B) Pollen tube growth in the style after a wild-type S3S13 plant was pollinated with pollen from T0 plant #13/S2*S3.
(C) Pollen tube growth in the style after a wild-type S13S13 plant was pollinated with pollen from a progeny plant, #27-BS-#1/S2*S2*, obtained by bud-selfing T0 plant #27/S2*S3. Scale bar = 1 mm in all microscopy images of aniline blue staining in (A), (B), and (C).
(D) Effect of a single amino-acid deletion (Q210) and a two-amino acid deletion (Q210 and L211) of S2-SLF1 on its ability to detoxify S3-RNase and S13-RNase. Seven amino acids of the wild-type S2-SLF1 in the region where deletions occur are shown for comparison. + indicates ability to detoxify S3-RNase and S13-RNase, and — indicates inability to detoxify these two S-RNases.