Figure 2.

The lepto1 Mutant Shows Normal Acquisition of Meiotic Fate but Disrupted Deposition of Meiotic Callose.

(A) and (B) Immunodetection of CENH3 (red) in PMCs of the wild type (A) and lepto1 (B). Chromosomes were stained with DAPI (light blue). Arrows indicate CENH3 signals in PMCs.

(C) and (D) Meiotic DNA replication occurred in lepto1 PMCs. Green fluorescence indicates EDU signals, and blue fluorescence indicates DAPI signals. Anthers containing four layers of somatic cells were selected for observation. Arrows indicate EDU signals, and EDU signals were detected in both wild-type and lepto1 PMCs.

(E) Transverse sections of wild-type anthers at various developmental stages stained with aniline blue. Arrows indicate aniline blue fluorescence signals.

(F) Transverse sections of lepto1 anthers at various developmental stages stained with aniline blue.

Bars = 5 μm.