. 2018 Sep 10;67(2):85–97. doi: 10.1369/0022155418798807

Table 2.

Primary Antibodies.

Antigen	Antibody	Source^(Reference)	Dilution/Concentration
Antigen	Antibody	Source^(Reference)	WB	IH^a
Filaggrin	clones AHF1-AHF27	Home made^30,31	0.5 µg/ml	5 µg/ml
Corneodesmosin	clones G36-19 and F28-27	Home made¹⁹	0.5 µg/ml	5 µg/ml
No specificity	clone MOPC21	Sigma-Aldrich	0.5 µg/ml	—
Keratin 10	clone RKSE60	ThermoFisher	—	1:100
Keratin 14	clone LL002	ThermoFisher	—	1:100

Abbreviations: AHF, anti-human filaggrin; WB, western blotting; IH, immunohistology.

Antigen retrieval was not necessary.