Fig. 5.

Dams fed a high fat diet for 29 weeks were more responsive to methacholine. From 4 weeks of age, female mice were fed either a low fat diet (LFD, n = 14) or high fat diet (HFD, n = 13). Female mice were mated with male mice, first after dams were fed either diet for 12 weeks, and secondly 1 week after the first litter of 2 week-old offspring were tested for lung function. After eating the LFD or HFD for 29 weeks, the dams had their lung function measured following an initial challenge with saline and then increasing doses of methacholine. In (a), airway resistance; in (b), tissue damping; and, in (c), tissue elastance are shown. Data are shown as mean ± SEM (*p < 0.05)