Table 4.

Offspring fed a high fat diet had increased body weights and lengths

Outcome	LFD	HFD
First pregnancy	n = 18	n = 8
Body weight (g)	5.8 ± 0.2	7.4 ± 0.8*
Body length (snout to vent) (mm)	48.0 ± 0.8	53.5 ± 1.4*
Body mass index (kg/m2)	2.48 ± 0.06	2.53 ± 0.17*
Second pregnancy	n = 33	n = 7
Body weight (g)	5.9 ± 0.1	8.9 ± 0.3*†
Body length (snout to vent) (mm)	49.1 ± 0.6	56.7 ± 1.0*†
Body mass index (kg/m2)	2.47 ± 0.04	2.77 ± 0.09*§

There was a significant effect of both diet (p < 0.001*) and pregnancy (p < 0.035†) on offspring body weight and length (2-way ANOVA) with no significant interactions (p < 0.299). There was a significant effect of diet only (p < 0.001§) but not pregnancy (p = 0.169) on offspring BMI (2-way ANOVA) with no significant interaction (p = 0.131)

From 4 weeks of age, C57Bl/6 J female mice (n = 28) were separated into 2 treatment groups and fed either a low fat diet (LFD, n = 14) or high fat diet (HFD, n = 14). Female mice were mated with male C57Bl/6 J mice after dams were fed either diet for 12 weeks. Three weeks later, offspring were born. Dams were re-mated 1 week after their first litters were removed at 2 weeks of age. All measures were obtained from offspring at 2 weeks of age. Data are shown as mean ± SEM