Fig. 4.

LOXL4 enhances cell-matrix adhesion and activates the FAK/Src pathway in HCC cells. a LOXL4-overexpressing and control cells were subjected to cell-matrix adhesion assay to collagen I (Col I), collagen IV (Col IV), laminin (LN), and fibronectin (FN). b LOXL4 knockdown and control cells were subjected to cell-matrix adhesion assay to Col I, Col IV, LN, and FN. c Western blotting analysis of phosphorylation of FAK and Src and total FAK and Src in LOXL4-overexpressing and control cells. d Western blotting analysis of phosphorylation of FAK and Src and total FAK and Src in LOXL4 knockdown and control cells. e Western blotting analysis of phosphorylation of FAK and Src and total FAK and Src in LOXL4-overexpressing and control cells upon treatment with vehicle or PP2 (10 μM) for 24 h. f LOXL4-overexpressing and control cells were subjected to cell-matrix adhesion assay to Col I, Col IV, and FN upon treatment with vehicle or PP2. g Cell migration potential was determined in LOXL4-overexpressing and control cells upon treatment with vehicle or PP2 according to Transwell assays (Scale bar: 100 μm). (* P < 0.05, ** P < 0.01)