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. 2018 Oct 23;7(2):285–288. doi: 10.1016/j.jcmgh.2018.10.008

Figure 1.

Figure 1

Characterization of iPSC-derived intestinal epithelial organoids. (A) Intestinal epithelial organoids derived from PSCs (iPSCo), terminal ileum organoids (TIo), or SC organoids (SCo) in microscopic brightfield view (upper panel), or immunofluorescent staining for epithelial cell adhesion molecule (epithelial cell adhesion molecule [EpCAM], green), actin (red), and nuclei (blue). Scale bars: 400 μm. (B) Expression of IEC-type marker as normalized counts from RNA sequencing data of the respective organoids. Bar shows means ± SD, n = 4–5 per group. (C) Immunofluorescent staining for enterocyte marker villin (VIL1, green), goblet cell marker mucin 2 (MUC2, green), enteroendocrine marker chromogranin A (CHGA, green), Paneth cell marker lysozyme (LYZ, red), and nuclear counterstain (blue), Scale bars: 100 um.