Fig. 7.

In vitro and in vivo determination of the release of IL-6. (A) In vitro IL-6 quantities in the supernatants of MSC⁻ and MSC⁺ cultures under basal MSCs is associated with a release of IL-6 conditions 24 h after transfection (with and without plasmid), and 48 h after LPS treatment for 24 h. Data are shown as mean ± SD (***p < 0.001, n = 9). The plasmid encoding the fluorescent protein eGFP (pmaxGFP) was used as the control. pEAAT₂ represents the YFP-EAAT₂-encoding construct (B) Serum levels of IL-6 in ischemic rats treated with vehicle, 9 × 10⁶ MSCs⁻, and 9 × 10⁶ MSCs⁺. Data are shown as mean ± SD (**p < 0.01, n = 3). Non-transfected MSCs (MSCs⁻); transfected MSCs (MSCs⁺); lipopolysaccharide (LPS).