FIGURE 3.

Detoxifying capacity of B. subtilis PTA-271 (Bs) toward the purified (R)-mellein (A–C) and (-)-terremutin (D–F) from N. parvum. Phytotoxin concentrations were determined as remaining percentages in the bacterial incubating media either: (A,D) daily form MS medium containing PTA-271 (Bs) at 10⁸ cfu/mL, or (B,E) 72 h post-exposure to two distinct bacterial densities 10⁴ and 2 × 10⁸ cfu/mL in MS, or (C,F) 72 h post-exposure to the two distinct incubating media Murashige-Skoog medium (MS) and MgSO₄ with Bs at 2.10⁸ cfu/mL. Data are means ± SD of three independent experiments, each with triplicates. The toxin controls (Ctrl) indicated none physicochemical disappearance. Phytotoxins were not detectable inside bacterial pellet. Vertical bars with different letters are significantly different (Multiple Comparison procedures with Tukey’s test, P < 0.05).