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. 2019 Jan 24;10:25. doi: 10.3389/fpls.2019.00025

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Copyright © 2019 Trotel-Aziz, Abou-Mansour, Courteaux, Rabenoelina, Clément, Fontaine and Aziz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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B. subtilis PTA-271 strongly primes PR2 gene in leaves of grapevine cuttings after infection with the N. parvum strain Np-Bt67. Twelve weeks old plantlets untreated or pretreated with PTA-271 were both infected with sterile PDA plugs (Control and Bs, respectively) or with mycelium plugs of Np-Bt67 (Ctl + Np-Bt67 and Bs + Np-Bt67, respectively). Transcript levels of defense-related genes were monitored by qRT-PCR in leaves at 4 days post infection. Results are from one representative replicate among three independent experiments showing the same trends. Different letters indicate significant differences. PR3 = class IV chitinase (chit4c); PR4 = PR-4 type protein; LOX9 = lipoxygenase 9; PR1 = pathogenesis-related protein 1; PR2 = class I β-1,3-glucanase; PR5 = thaumatin-like protein; GST1 = glutathione-S-transferase 1; PR10 = pathogenesis-related protein 10; PAL = phenylalanine ammonia lyase; STS = stilbene synthase; NCED2 = 9-cis-epoxycarotenoid dioxygenase 2.