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. 2019 Jan 24;7:1. doi: 10.3389/fcell.2019.00001

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Copyright © 2019 Lim and O’Neill.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Morphology of splenic stromal fractions. Stromal cells were isolated from murine spleen using collagenase treatment, stained with antibody and sorted flow cytometrically. Cells were cultured and photographed after 28 days using a camera attached to an inverted phase microscope. Stromal fractions sorted on the basis of a single marker were tested for in vitro growth. (A) CD29⁺, (B) Sca-1⁺, (C) CD140a⁺, (D) CD105⁺, (E) gp38⁺, (F) ERTR7⁺, (G) CD51⁺ and (H) Thy1.2⁺.