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Expression of hematopoietic factors by spleen stromal fractions. Gene expression in 5G3 stroma was compared with several sorted stromal fractions of spleen using qRT-PCR. Data represent the mean ± SE of gene expression measured as fold change relative to the Actb (b-actin) housekeeping gene for four replicated samples. Expression of Cxcl12 and Scf by all stromal fractions tested was not significantly different from 5G3 (p < 0.05).
