. 2019 Jan 24;7:1. doi: 10.3389/fcell.2019.00001

Table 4.

Characterization of specific subsets amongst the spleen stromal fraction.

(A) Subset representation.
Subset^a	Designation^b	% cells^c

Sca-1^hiThy1.2^-CD105⁺CD51^loCD140a^-	P8	0.17 ± 0.068
Sca-1^loThy1.2^loCD105⁺CD51⁺CD140a⁺	P9	0.06 ± 0.021
Sca-1^loThy1.2^-CD105⁺CD51^loCD140a^lo	P10	0.52 ± 0.190
Sca-1^loThy1.2^-CD105^-CD51⁺CD140a^lo	P11	0.04 ± 0.006
Sca-1^-Thy1.2^loCD105^loCD51⁺CD140a^lo	P12	0.03 ± 0.007
Sca-1^-Thy1.2^-CD105⁺CD51⁺CD140a^lo	P13	0.06 ± 0.017

(B) Phenotype of cells cultured out of stromal subsets.

Subset^b	Cell number plated^d	Phenotype of 28 day stroma^e

P8	(2.8 ± 0.5).10⁴ (n = 5)	No growth
P9	(7.6 ± 1.5).10³ (n = 5)	Sca-1⁺gp38⁺CD51^loCD105^-CD29⁺ER-TR7^-CD140a^-Thy1.2⁺
P10	(9.8 ± 1.6).10³ (n = 5)	Sca-1⁺gp38⁺CD51^-CD105^-CD29⁺ER-TR7^-CD140a^-Thy1.2⁺
P11	(1.3 ± 9.6).10² (n = 5)	No growth

^aThe stromal the fraction of neonatal 6-day old spleen was isolated by collagenase digestion, stained for markers, and then delineated through flow cytometric gating of CD45^- cells.^bSubset designation is shown in Figure 5.^cData reflect mean ± SE (n = 3).^dSubsets were cultured for 28 days to determine capacity to form a monolayer.^eMonolayers were trypsinised and cells stained with antibodies to determine phenotype flow cytometrically.