(B) Proportion of myeloid subsets produced in stromal co-cultures. | ||||||
% L-DCa,b |
% cDC-likea,b |
% Myeloida,b |
||||
1st Experiment | 2nd Experiment | 1st Experiment | 2nd Experiment | 1st Experiment | 2nd Experiment | |
gp38+ | 81.0 | 86.9 | 0.14 | 0 | 18.9 | 13.1 |
CD140a+ | 75.1 | 65.6 | 0.08 | 0.03 | 24.8 | 34.3 |
CD105+ | 87.9 | 39.3 | 0 | 0.02 | 12.2 | 60.5 |
P9 | 58.5 | 47.8 | 0.03 | 0 | 41.2 | 52.6 |
P10 | 52.4 | 47.8 | 0.01 | 0.04 | 47.6 | 52.1 |
5G3c | 90.5 ± 1.77 | 2.63 ± 1.42 | 7.02 ± 0.41 | |||
aSee legend to table above. Data are shown for 2 independent experiments for test stroma.bFlow cytometry was used to identify myeloid cells produced in 21 day co-cultures through staining with CD11b, CD11c, MHC-II and F4/80. Data shown represent proportion of subset relative to total myeloid subset defined as CD11c+ and/or CD11b+ cells.cData for 5G3 is shown as mean ± SE (n = 5).