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. Author manuscript; available in PMC: 2019 Jan 31.
Published in final edited form as: Pediatr Res. 2014 Dec 18;77(3):406–415. doi: 10.1038/pr.2014.208

Figure 4. In situ hybridization of zonal markers of articular cartilage.

Figure 4

Formalin fixed, decalcified sections of 1-wk old mouse tibial cartilage were hybridized to DIG-labeled riboprobes, producing a purple color and were visualized by scanning the slides with a ScanScope CS digital scanner under bright field microscopy. Left panel, Mason-trichrome stained sections; middle panel, in situ hybridization without counterstaining; right panel, high magnification views taken from within the rectangular area indicated in the corresponding middle panel. SZ, superficial zone; MZ, middle zone; DZ, deep zone; RZ, resting zone; PZ, proliferative zone; HZ, hypertrophic zone.