Formalin fixed, decalcified sections of 1-wk old mouse tibial cartilage were hybridized to DIG-labeled riboprobes, producing a purple color and were visualized by scanning the slides with a ScanScope CS digital scanner under bright field microscopy. Left panel, Mason-trichrome stained sections; middle panel, in situ hybridization without counterstaining; right panel, high magnification views taken from within the rectangular area indicated in the corresponding middle panel. SZ, superficial zone; MZ, middle zone; DZ, deep zone; RZ, resting zone; PZ, proliferative zone; HZ, hypertrophic zone.