Table 4.
Genetic Signatures and Single-Nucleotide Polymorphism in Culex torrentium, Culex pipiens, and Their Hybrids
| SNP position no. | Cx. torrentium sequence | Cx. pipiens sequence | Hybrid sequence |
|---|---|---|---|
| 695 | AGGATTT | AGGGTTT | #101 – AGGGTTT |
| 704 | TGTATGA | TGTTTGA | |
| 821 | TACCCTT | TACTCTT | #39 – TACTCTT |
| #105 – TACTCTT | |||
| 842, 848, 851 | AAATTATACTCCCGCTT | AAACTATACACCTGCTT | |
| 899 | AGGTTTA | AGGATTA | |
| 947 | TCACGAT | TCATGAT | #31 – TCATGAT |
| #33 – TCATGAT | |||
| #56 – TCATGAT | |||
| #92 – TCATGAT | |||
| 965, 971 | TGTCGCTCACTT | TGTTGCTCATTT | #31–TGTTGCTCACTT |
| #92–TGTTGCTCACTT | |||
| #95–TGTTGCTCACTT | |||
| 1023 | TTTGTTC | TTTATTC | |
| 1034 | ATACCCT | ATATCCT | |
| 1061 | AAATCCT | AAACCCT | |
| 1077, 1078 | AAAGGCTCAA | AAAGATTCAA | #1 – AAAGGTTCAAa |
| #77 – AAAAGTTCAA | |||
| #89 – AAAGACTCAA | |||
| #93 – AAAGATTCAA | |||
| 1124 | CCTCAA | CCCACAA | |
| 1139 | AGGTTTA | AGGATTA | |
| 1154, 1163 | ACCTCGACGATACTCT | ACCACGACGATATTCT | |
| 1184 | TTATTTA | TTACTTA | |
| 1199, 1209 | TATCGTTTCATCACTAG | TATTGTTTCATCATTAG |
Underlined bold letters indicate the nucleotide found in (the COI gene of) Cx. torrentium, Cx. pipiens and hybrid mosquitoes. The SNP position and mosquito number is also specified for each hybrid mosquito.
A conserved nt change among the captured mosquitoes (not toward Cx. pipiens or Cx. torrentium): was found in positions 732 (A→G) #48, 57, 95, 104, 108, 109, 114, 115, 117, 118.
a
Differences in nt sequence between Cx. pipiens and Cx. torrentium; and also numbers 2–3, 5, 29, 32, 34–37, 40–41, 57, 61–64, 67, 68, 70, 76, 80, 84, 85, 88, 95, 96, 99, 104, 105, 109–111, 113–115, 117, 118.
SNP, single-nucleotide polymorphism.