Skip to main content
. 2018 Oct 30;24:7750–7758. doi: 10.12659/MSM.911585

Figure 4.

Figure 4

MiR-492 expression was directly regulated by p53. (A) ChIP analysis of p53 enrichment at the promoter region of miR-492 was performed with SiHa and HeLa cells. The chromatin segments bound to p53 or control IgG were purified and further analyzed by semi-quantitative PCR with primers surrounding the p53 BS. (B) p53 BS transcriptional activity with or without 5-FU induction was validated by dual-luciferase reporter assays. pGL3-basic construct harboring wild-type or mutant p53 BS or the control vector was transfected into SiHa cells together with pRL-SV40 as an internal control with or without 300 μM 5-FU for 20 hours. Data were represented as means ±SD from 3 independent experiments, * P<0.05. ChIP – chromatin immunoprecipitation; 5-FU – 5-fluorouracil.