Fig. 4.

Kdm4d knockdown effectively suppresses HSC activation in vitro. (a) Western blotting analysis of Kdm4d knockdown efficiency in activated C57BL/6J mice primary HSCs. (b) Effects of Kdm4d knockdown on the expression of pro-fibrogenic genes (Acta2, Col1a1, and Vim) were examined by qRT-PCR. (c) Representative immunofluorescence images of primary HSCs from sh-Ctrl and sh-Kdm4d mice. (d) Collagen gel contraction assay of primary HSCs from sh-Ctrl and sh-Kdm4d mice (n = 3 for each group). Statistical analysis of collagen area/dish area was shown in the right panel. (e) Representative migration images of primary HSCs from sh-Ctrl and sh-Kdm4d mice (n = 5 for each group). Statistical analysis of cell number per field was shown in the right panel. Data were presented as mean ± SD, *p < .05, and **p < .01.