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. 2019 Jan 25;14:835–849. doi: 10.2147/IJN.S184013

Figure 2.

Figure 2

Electrochemical behaviors of the biosensor.

Notes: (A) CV response of (a) bare GE, (b) MEA/GE, (c) rGO-PAMAM-Fc/MEA/GE, (d) (rGO-PAMAM-Fc)2/MEA/GE, (e) (rGO-PAMAM-Fc)3/MEA/GE, (f) (rGO-PAMAM-Fc)4/MEA/GE, (g) (rGO-PAMAM-Fc)5/MEA/GE, (h) Au NPs/(rGO-PAMAM-Fc)5/MEA/GE, (i) ChOx&ChEt/Au NPs/(rGO-PAMAM-Fc)5/MEA/GE in [Fe(CN)6]3−/4− solution from −0.4 to 0.8 V with a scanning rate of 100 mV/s. (B) EIS of (a) bare GE, (b) MEA/GE, (c) rGO-PAMAM-Fc/MEA/GE, (d) (rGO-PAMAM-Fc)2/MEA/GE, (e) (rGO-PAMAM-Fc)3/MEA/GE, (f) (rGO-PAMAM-Fc)4/MEA/GE, (g) (rGO-PAMAM-Fc)5/MEA/GE, (h) Au NPs/(rGO-PAMAM-Fc)5/MEA/GE, (i) ChOx&ChEt/Au NPs/(rGO-PAMAM-Fc)5/MEA/GE in PBS containing 5 mM Fe(CN)63−/4− at 0.24 V (vs SCE) with a frequency range of 0.1–100 kHz. (C) Current response toward 5 µM cholesterol by LSV with the different modifications, including bare GE, ChOx&ChEt/rGO-PAMAM-Fc/GE, ChOx&ChEt/Au NPs/rGO-PAMAM/GE, ChOx&ChEt/Au NPs/PAMAM-Fc/GE, ChOx&ChEt/Au NPs/PAMAM/GE and ChOx&ChEt/Au NPs/(rGO-PAMAM-Fc)/GE. Error bars represent the standard error of the mean (n=3 electrodes).

Abbreviations: CV, cyclic voltammetry; EIS, electrochemical impedance spectroscopy; Fc, ferrocene; GE, gold electrode; LSV, linear sweep voltammetry; NPs, nanoparticles; PAMAM, polyamidoamine; rGO, reduced graphene oxide; SCE, saturated calomel electrode.