Figure 5.

Optimization of the determining conditions.

Notes: (A) Optimization of the number of layers of rGO-PAMAM-Fc nanomaterial film on GE. (B) Effect of different molecular generations of PAMAM in rGO-PAMAM-Fc nanoparticles toward cholesterol. (C) Effect of temperature of deposition reaction on the current response to cholesterol. (D) Effect of electrodeposition time of Au NPs on the modified electrode. (E) Effect of glycine buffer pH on the current response to cholesterol. (F) Effect of concentration of Ag⁺ in the glycine buffer. (G) Effect of time of deposition reaction on the current response to cholesterol. The current response was recorded using LSV measurements in 0.1 mol/L HNO₃ solution containing 0.6 mol/L KNO₃ from −0.2 to 0.6 V at 0.1 V/s scanning rate. Error bars are the standard error of the mean (n=3 electrodes).

Abbreviations: Fc, ferrocene; GE, gold electrode; LSV, linear sweep voltammetry; NPs, nanoparticles; PAMAM, polyamidoamine; rGO, reduced graphene oxide.