Figure 1.

System Overview

The constructs used in the study are schematically indicated. The wavy line refers to the myristoyl group attached to the N terminus. The structure of the SH3 domain, with the three loops and the two tryptophan residues, is shown in the right. The domain structure of the c-Src protein is shown on the top, and the sequence of the wild-type MyrUSH3 is indicated at the bottom. The myristoylated constructs contained a His₆ tag after the SH3 domain. The non-myristoylated constructs contained an additional GAMA tetrapeptide that arises from cloning instead of the N-terminal glycine.