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. 2019 Jan 14;12:182–193. doi: 10.1016/j.isci.2019.01.015

Figure 4.

Figure 4

Cell Death Assays Validate AKTM Predictions

(A) Percentage cell death in untreated (control) HCT116 cells.

(B) Percentage cell death in HCT116 cells treated with 2.5 μg/mL cycloheximide.

(C) Percentage cell death in HCT116 cells treated with 50 ng/mL TRAIL and 2.5 μg/mL cycloheximide.

(D) Time to 50% cell death in HCT116 cells treated with 50 ng/mL TRAIL and 2.5 μg/mL cycloheximide. BAX−/− cells reached 50% cell death significantly later than in wild-type (p < 0.05), PTEN−/− cells did not significantly differ from wild-type, and XIAP−/− cells reached 50% cell death significantly earlier (p < 0.01).

Data in (A–D) represent means ± SD from three independent biological repeats. SDs are represented by shaded areas in (A–C) and by error bars in (D). * in (D) indicates a mean significantly different from that of the wild-type cell line with p ≤ 0.05 (Welch two-sample t test); ** signifies p ≤ 0.01. Detailed statistical results are displayed in Table S5.