Skip to main content
. 2019 Jan 30;4(1):e00696-18. doi: 10.1128/mSphere.00696-18

TABLE 2.

Primers

Primer Sequence (5′–3′) Purpose Reference
or source
Tn7 F 5′-CAGCATAACTGGACTGATTTCAG-3′ Verify integration of
transposon into
chromosomal glmS
locus		 48
glmS R 5′-TGCTCAAGGGCACTGACG-3′ Verify integration of
transposon into
chromosomal glmS
locus		 48
PompA-dsRed F 5′-ACGTTCTCGGAGGAGGCCATCAACGCAACAAAAGAAACTGC-3′ Amplification of
N824_RS25200
promoter to join
with dsRed gene		 This study
PompA R 5′-TATGGTACCAGTCATCTAGGCGGCTGTAG-3′ Amplification of
N824_RS25200
promoter to join with
dsRed gene; includes
KpnI-site for inserting
into pHimarEm1		 This study
dsRed F 5′-TACTCAGGAGAGCGTTCACC-3′ Amplification of
sRed gene with no
promoter, to join with
V48 N824_RS25200
promoter by SOE PCR		 This study
dsRed R 5′-GCAGTTTCTTTTGTTGCGTTGATGGCCTCCTCCGAGAACGT-3′ Amplification of
dsRed gene with no
promoter, to join with
V48 N824_RS25200
promoter by SOE PCR;
includes KpnI-site for
inserting into pHimarEm1		 This study
pHimar KpnI-flank F 5′-CTGCCCTGCAATCGACCTCG-3′ Verify ligation of dsRed
into pHimarEm1		 This study
pHimar KpnI-flank R 5′-CAGATAGCCCAGTAGCTGAC-3′ Verify ligation of dsRed
into pHimarEm1		 This study
erm F 5′-CCGCACCCAAAAAGTTGCAT-3′ Verify integration of
transposon into
V48 chromosome		 This study
erm R 5′-GACAATGGAACCTCCCAGAA-3′ Verify integration of
transposon into
V48 chromosome		 This study
ARB1 5′-GGCCACGCGTCGACTAGTACNNNNNNNNNNGATAT-3′ Find location of
transposon integration
in V48 chromosome		 52
ARB6 5′-GGCCACGCGTCGACTAGTACNNNNNNNNNNACGCC-3′ Find location of
transposon integration
in V48 chromosome		 52
ARB2 5′-GGCCACGCGTCGACTAGTAC-3′ Find location of
transposon integration
in V48 chromosome		 52
Himar Arb1 (TnExt) 5′-GTGTTGTTCCAGTTTGAGATC-3′ Find location of
transposon integration
in V48 chromosome		 This study
Himar609 Arb2 (TnInt) 5′-TGGGAATCATTTGAAGGTTGG-3′ Find location of
transposon integration
in V48 chromosome		 23