Fig. 5. Photoactivation of caged GA4in vivo: (A) structure of caged GA4. (B–D) Dark stability and photoactivation of 4in vivo. 4 days old pRGA:GFP-RGA Arabidopsis seedlings (n = 3) were treated with paclo (2 μM) overnight and incubated with 4 at indicated concentrations for 15 minutes. (B) Seedlings were kept in the dark until imaged at indicated times. (C) Seedling roots were irradiated using a confocal lamp (365/10 nm, 2 mW cm–2) for indicated times. Root tips were imaged 90 minutes later. Error bars represent SEM. *Statistical significance (one-way ANOVA with Tukey correction, p < 0.05) from the T0 of 0 μM 4. (D) Representative images of plants treated with 5 μM 4 in (C). Red: PI. Green: GFP-RGA.
