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A: Phenotypic detection of secreted aspartyl protease activity in C. albicans isolates by culturing on bovine serum agar (BSA) agar and incubated at 28°C for 7 days and measuring the clearing zone around the growing colony, B: Detection of biofilm activity in C. albicans isolates after biofilm growth on microtiter plate containing Sabouraud broth (SDB) for 48 hours and measure the absorbance at 540 nm after staining with crystal violet.
