Fig. 1.

Principal component analysis of whole-transcriptome data derived from multiple differentiated human islet-like cell models. Data include all stages from our current differentiation protocol (Current), the most mature stage of a previously published differentiation protocol (Previous) [10], and cells derived via in vivo maturation by Xie and colleagues (Xie) [14]. The first two principal components (PC1, PC2) have been calculated using normalised gene counts for all stages of all three studies and corrected for batch effects. DE, definitive endoderm; GT, primitive gut tube; PF, posterior foregut; PE, pancreatic endoderm; EP, endocrine precursor; EN, endocrine-like cells; BLC, beta-like cells. Stages shown from the current study are iPSC, DE, GT, PF, PE, EP, EN and BLC. The stage shown from the previously reported study [10] is EN. The stage shown from Xie and colleagues’ in vivo maturation study [14] is ‘Matured in vivo’