a-c, Immunoblot analysis of EGFR, MAPK and FAK phosphorylation during WR and ΔVGF infections. d, Plaque formation in the presence of VGF signalling inhibitors. e, Diameter of plaques from d. f, Single cell tracking of VACV plaque formation in the presence of EGFR, MEK or FAK inhibitors (24 - 48 hpi). Tracks are colour-coded by hpi. g, h, The radial velocity and directional migration efficiency of cells migrating from the centre of plaques in f. Data represent 3 or more biological replicates (a-h). Images are representative of 3 biological replicates (a-d, f). Lines represent means of 100 plaques per condition/replicate (e). Scale bar=500 μm (f). Bars represent means + SEM of n=5 plaques per condition/replicate (g, h). Unpaired t-test was applied (**** P< 0.0001). See Supplementary Table 1 for exact statistics.