Fig. 1.

Effects of TBBPA on MMP-9 expression and transcriptional activity. Cell viability was estimated by the (A) MTT and (B) LDH release assays. (C) Cells were treated with 1, 5, or 10 μM TBBPA for 24 hr. Cells were then lysed and total RNA was prepared to analyze MMP-9 gene expression. (D) Cells were transfected with MMP-9-Luc for 4 hr and then treated with 1, 5, or 10 μM TBBPA for 24 hr. Cells were then harvested and assayed for luciferase activity. (E) Effect of TBBPA on MMP-9 activity levels in MCF-7 cells. Cells were treated with 1, 5, or 10 μM TBBPA for 24 hr. MMP-9 activity in MCF-7 cells was determined by gelatin zymography and Western blot analysis. Each bar represents the mean ± SD calculated from three independent experiments. *Significantly different from the control at p < 0.01.