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. 2019 Jan 31;14(1):e0211719. doi: 10.1371/journal.pone.0211719

Fig 4. Comparing reactivity of canine sera using rK39 and rKDDR ELISA assays and the EIE-LVVLC kit.

Fig 4

Reactivity of canine sera assessed using EIE-LVC kit (A-B), analyzed with two cut-off values; rK39 (C); and rKDDR (D). The different ELISA assays were performed using sera from the following groups of dogs: NC, negative control (n = 81); Babesia, dogs with babesiosis to assess cross-reactivity (n = 15); Tc, dogs with Chagas disease to evaluate cross-reactivity (n = 15); and CVL, dogs with canine visceral leishmaniasis (n = 96). The y-axis shows absorbance at 492 nm. The x-axis shows different groups of canine sera. The red line shows the lower limit of positivity (cut-off). The index above each column in the plot indicates the percentage of points that are above the cut-off. The ROC curve was used to determine the cut-off for each test. *Cut-off obtained by the ROC curve; #Cut-off obtained according to manufacturer’s manual.