Table 1. Sensitivity, specificity, positive and negative predictive value, and accuracy of ELISA assays in diagnosing canine sera.

ELISA Test	Cut-off	Parametersα
		Sensitivity (%)	95% CI	Specificity (%)	95% CI	PPV (%)	NPV (%)	AC (%)
rKDDR*	0.111	88.54	80.42–94.14	97.30	92.30–99.44	96.70	90.76	93.24
rK39*	0.131	78.13	68.53–85.92	90.09	82.96–94.95	87.20	82.64	84.54
EIE-LVC Kit*	0.162	93.75	86.89–97.67	86.49	78.69–92.23	84.90	94.05	89.37
EIE-LVC Kit#	0.045	98.96	94.33–99.97	52.25	42.56–61.82	64.18	98.30	73.91

Abbreviations: (CI) confidence interval; (PPV) positive predictive value; (NPV) negative predictive value; (AC) accuracy

^α The calculation of the parameters was performed using all dog serum samples of this work (NC, n = 81; Babesia, n = 15; Tc, n = 15; CVL, n = 96)

* Cut-off calculated based on ROC curve

^# Cut-off calculated according to the manual of EIE-LVC kit.