Table 2. Results of diagnostic agreement between rKDDR-ELISA, rK39-ELISA, and the EIE-LVC kit assessed using canine sera.
| ELISA Test | AUC | 95% CI | tP | tN | FP | FN | κα | 95% CI | Agreementβ |
|---|---|---|---|---|---|---|---|---|---|
| rKDDR* | 0.954 | 0.922–0.985 | 85 | 108 | 3 | 11 | 0.863 | 0.794–0.932 | Very good |
| rK39* | 0.901 | 0.869–0.949 | 75 | 100 | 11 | 21 | 0.687 | 0.588–0.786 | Good |
| EIE-LVC Kit* | 0.951 | 0.924–0.978 | 90 | 95 | 16 | 6 | 0.788 | 0.704–0.871 | Good |
| EIE-LVC Kit# | NA | NA | 95 | 58 | 53 | 1 | 0.494 | 0.393–0.595 | Moderate |
Abbreviations: (AUC) area under the curve; (CI) confidence interval; (tP) test positive; (tN) test negative; (FP) false positive; (FN) false negative
α To calculate Kappa index, all canine serum samples was used (NC, n = 81; Babesia, n = 15; Tc, n = 15; CVL, n = 96)
β Parasitological diagnostic assay was the gold standard test for calculating the Kappa index
* Cut-off was calculated based on ROC curve
# Cut-off was calculated according to the manual of EIE-LVC kit.