Table 3. Results of sensitivity, specificity, positive and negative predictive value, and accuracy of the ELISA assays assessed using human sera.

ELISA Test	Cut-off	Parametersα
		Sensitivity (%)	95% CI	Specificity (%)	95% CI	PPV (%)	NPV (%)	AC (%)
rKDDR*	0,242	92.86	85.10–97.33	100.00	92.60–100.00	100	88.88	95.65
rK39*	0,204	90.48	82.09–95.80	97.92	88.93–99.95	98.70	85.45	93.61

Abbreviations: (CI) confidence interval; (PPV) positive predictive value; (NPV) negative predictive value; (AC) accuracy

^α The calculation of the parameters was performed using all human serum samples of this work (NC, n = 31; Tc, n = 17; HVL, n = 84)

* Cut-off calculated based on ROC curve