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. 2019 Jan 18;15(1):e1007507. doi: 10.1371/journal.ppat.1007507

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© 2019 Gurung et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 10 — Pan flavivirus immunofluorescence (IF; Red: flavivirus; Blue: DAPI) staining in placenta from control (A), and three placenta from ZIKV infected dams that ZIKV RNA+ in placenta (B, Dam 3, 14 dpi; C, Dam 3, 14 dpi; D, Dam 4, 21 dpi). The most intense IF was observed in the placenta of Dam 2 (B) in which there was fetal death. ZIKV IF was observed in syncytiotrophoblast (arrows) in Dam 2 and 3. Arrowheads in B indicate IF in the villous cores which could be either stromal or endothelial cells. A less intense IF pattern was observed in Dam 4 (D) in the overlying syncytiotrophoblast which is covering the underlying cytotrophoblasts, despite widespread vertical transfer of ZIKV to the fetus.